Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| A. hydrophila | ||
| W1 | wild‐type strain | |
| M01 ~ M332 | mini‐Tn10Km insertion mutant (SmR KmR) | This study |
| M196 | rbsR: mini‐Tn10Km (SmR KmR) | This study |
| MC196 | WM196 complemented with pACYC184‐rbsR (CmR) | This study |
| Escherichia coli | ||
| SM10 | thi thr leu tonA lacY supE recA RP4‐2‐Tc::Mu::Km (λpir)F‐, 80dlacZ ΔM15, Δ(lacZYA‐argF) U169, deoR, recA1 | (Herrero et al., 1990) |
| E. coli DH5α | endA1, hsdR17 (rK−, mK+), phoA, supE44, λ‐, thi‐1, gyrA96, relA1 | |
| Plasmid | ||
| pMD18‐T | Cloning vector (ApR) | Takara |
| pLOF/Km | Tnl0‐based delivery plasmid with (KmR ApR); | (Herrero et al., 1990) |
| pACYC184 | (CmR TcR) | provided by Prof. Nie |
| pACYC184‐rbsR | pACYC184 derivative containing 1,005 bp fragment of rbsR putative promoter and ORF(CmR) | This study |