Figure 3.

Fold change of rpoE, groEL, htpG, bopA, bopE, and bipD genes in Burkholderia pseudomallei K96243 grown in Luria–Bertani (LB) broth containing 0, 150, and 300 mmol L⁻¹ NaCl. RNA of B. pseudomallei grown in LB broth with different NaCl concentrations for 6 hr was used for determination of gene expression by quantitative real‐time RT‐PCR using the Brilliant II SYBR ^® Green QPCR Master Mix, one step (Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer's recommendation. Relative mRNA levels were determined by fold changes in expression, calculated by 2^−ΔΔCT. 23S rRNA gene was used for normalization. Error bars represent the standard deviation of the means for experiments performed in triplicate