Figure 1.

Summary diagram of KinA and its truncated mutants. The N‐terminal domain is subdivided into three PAS domains (PAS‐ABC). The C‐terminal domain is subdivided into DHp (dimerization and histidine phosphotransfer) domain and CA (catalytic ATP‐binding) domain (Eswaramoorthy et al., 2009). Domain boundaries indicate the amino acid residue numbers from N‐terminus. Positions of cross‐linkable cysteine residues with bis‐maleimidohexane (BMH) are indicated in the N‐terminal domain. The histidine phosphorylation site is located at His405 as indicated. As a summary of this study, autokinase activity, tetramer (4‐mer) formation, and reverse phosphotransfer activity (reverse reaction from Spo0F~P to KinA_C) are scored with a plus sign or a minus sign to denote detectable or not, respectively