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. 2017 Aug 8;199(17):e00177-17. doi: 10.1128/JB.00177-17

TABLE 2.

Preexisting polymorphisms distinguishing DG1 from DSM639

Position Nucleotide(s) found in straina:
ORF (Saci) Effect
DSM639 DG1
6661 A C _0010 L→R
81843 TT TC _108 Stop→R
304011b T A _0360 Synonym
304038b T C _0360 Synonym
304052b T A _0360 M→K
329548 TT CT Intergenic
348066b G A _0406 T→M
348082b T G _0406 N→H
348226 G A _0406 Q→stop
375745 (A)8 (A)9 _0437 Frameshift mid-ORF
836667 TT TTT _1037 Frameshift mid-ORF
1298723 G A _1521 A→T
1674986 (59bp)5 (59bp)6 CRISPR2 SR copy no.
1700422 G A _1904 Q→stop
1837115b TTTCCT TCTCAG _2023 L→V plus synonym
1837131b CGGC TGGT _2023 Synonym
1837160b TT TC _2023 Synonym
1837173b TTTA CCTT _2023 Synonym
1917860 G A _2099 Synonym
1937684 G A _2116 A→V
2026424 C T _2189 A→T
2027418 (T)7 (T)5 Intergenic
a

59bp refers to TAGGAGGAAAATATAGGAATATAAAGATAGTACTAGTAATAACGACAAGAAACTAAAAC.

b

Clustered polymorphism; the second cluster is detailed in Fig. 5.