Figure 1.
Plasmid content of the Salmonella isolate from swine, Italy 2013 (n = 1) and its transformant 3445T and map of the pMCR plasmid carrying the mcr-4 gene
Left panel: M: 1 Kb Plus DNA ladder (Invitrogen, Italy). EcoRV-restricted plasmids purified from the R3445 Salmonella strain and the pMCR plasmid purified from the 3445T strain obtained by transformation (indicated by the arrow). Among the five ColE-like plasmids predicted by WGS, the pMCR ColE10 was estimated to be 8.7 kb, three copies per cell, while the other four ColE1 ranged from 7.0 to 4.0 kb in size and were estimated at more than 10 copies per cell.
Right panel: map of pMCR in the R3445 genome (http://wolfe.ucd.ie/GenomeVx/). The sequence of this plasmid is released at the NCBI GenBank under accession number MF543359.
