Table 2. Primers used in this study for the detection of the new mcr-4 gene.
Primer name | Sequence | Target | Amplicon size |
---|---|---|---|
Mcr-4 FW | ATTGGGATAGTCGCCTTTTT | primers for the screening of the new mcr-4 gene | 487 bp |
Mcr-4 RV | TTACAGCCAGAATCATTATCA | ||
Mcr-4 ext FW | ATCTGTTAAGTTTGTTGGTGAC | external primers to amplify the complete mcr-4 gene | 1,820 bp |
Mcr-4 ext RV | TGAGAGCTAAATGTAACAATAGA | ||
ColE10_repBF | TAAAGCATCGTGTAGTGTTTT | primers for the detection of the repB of the ColE10 plasmid | 420 bp |
ColE10_repBR | ATATAATCTGGAACATGTTAAG | ||
IS5FW | CATGACCTCAATCAGCTGG | In combination with ColE10_repBF, used for PCR-based closure of pMCR | 4,588 bp |
IS5 RV | TTTACTGAGATCTCTCCCAC | In combination with Mcr-4-RV, used for PCR-based closure of pMCR | 2,143 bp |