Figure 5. Detection of Gapmer MGC10 in mouse kidney by in situ hybridization.

24 h after subcutaneous injection (5 mg kg⁻¹) of MGC10, red fluorescence signals (by in situ hybridization of renal sections with a Texas Red conjugated complementary probe) were detected in tubular and glomerular areas (mostly in cytoplasm). However, after 48 h, nuclear accumulation of MGC10 was observed and the signals were more enriched in nuclei at 72 h after injection. Blue 4,6-diamidino-2-phenylindole signals represent nuclear regions. Weak background was detected in the kidneys of mice injected with vehicle (PBS) as control. Scale bar, 20 μm.