Table-2.
Diplex PCR: Primers sequences, target genes, amplicon sizes, and cycling conditions.
| Target gene | Primers sequences | Amplified segment (bp) | Primary denaturation | Amplification (35 cycles) | Final extension | Reference | ||
|---|---|---|---|---|---|---|---|---|
| Secondary denaturation | Annealing | Extension | ||||||
| Stx1 | ACACTGGATGATCTCAGT GG | 614 | 95°C 3 min | 94°C 60 s | 59°C 45 s | 72°C 90 s | 72°C 10 min | [18-20] |
| CTGAATCCCCCTCCATTA TG | ||||||||
| Stx2 | CCATGACAACGGACAGCAGTT | 779 | ||||||
| CCTGTCAACTGAGCAGCACTTTG | ||||||||
| hylA | ACGATGTGGTTTATTCTG GA | 165 | 53°C 45 s | |||||
| CTTCACGTGCCATACATAT | ||||||||
| eae A | GACCCGGCA ACAAGCATA | 384 | ||||||
| AGC | ||||||||
| CCACCTGCAGCAACAAGAGG | ||||||||
PCR=Polymerase chain reaction