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Details of the primers used for the detection of stχ1, stχ2, eaeA and hly A genes.
| S. No | Primer | Sequence (5’---- 3’) | Target gene | Expected amplicon size (bp) |
|---|---|---|---|---|
| 1 | Stχ1F | ATAAATCGCCATTCGTTGACTAC | stχ1 | 180 |
| Stχ2 R | AGAACGCCCACTGAGATCATC | |||
| 2 | Stχ2 F | GGCACTGTCTGAAACTGCTCC | stχ2 | 254 |
| Stχ2 R | TCGCCAGTTATCTGACATTCTG | |||
| 3 | eaeA F | GACCCGGCACAAGCATAAGC | eaeA | 384 |
| eaeA R | CCACCTGCAGCAACAAGAGG | |||
| 4 | hlyA F | GCATCATCAAGCGTACGTTCC | hlyA | 534 |
| hlyA R | AATGAGCCAAGCTGGTTAAGCT |
