FIG 8.

Motion simulations of V2i MAb access to the V1V2 domain. Ensembles of conformations from the simulations of JR-FL WT and from the K421N mutant Env trimers were analyzed to determine binding accessibility for two V2i-specific MAbs: 697 and 830A. Each dot represents a conformation, colored according to its energy (E) relative to the lowest energy conformer in the ensemble. The x and y coordinates show the numbers of atoms that would overlap other parts of the spike if the MAb were bound to its epitope on the V1V2 domain. Access to an epitope is unobstructed if the overlap is close to zero.