FIG 3.

Tonsil TFR cells are highly permissive to R5-tropic HIV as defined by Foxp3 expression. Fresh, disaggregated tonsil cells were spinoculated with R5-tropic GFP reporter virus for 2 h at room temperature and analyzed by flow cytometry after 48 h. The following T cell subsets were first defined by surface and intranuclear proteins: EF (CD3⁺ CD8⁻ CXCR5⁻ Foxp3⁻); EF Treg (CD3⁺ CD8⁻ CXCR5⁻ Foxp3⁺); TFH (CD3⁺ CD8⁻ CXCR5⁺ Foxp3⁻); TFR (CD3⁺ CD8⁻ CXCR5⁺ Foxp3⁺); GC TFH (CD3⁺ CD8⁻ CXCR5⁺ PD1⁺ Foxp3⁻); GC TFR (CD3⁺ CD8⁻ CXCR5⁺ PD1⁺ Foxp3⁺) (A) Percent p24 Ag⁺ cells. (B) MFI of p24 Ag. Statistical significance was determined using repeated-measures one-way ANOVA. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Only pairwise comparisons of interest are shown. The overall P value was <0.05 (by ANOVA) for all.