FIG 5.

R5-tropic permissivity of seronegative LN cells and sorted tonsil cells. (A) Cryopreserved seronegative LN cells were thawed and spinoculated with R5-tropic or X4-tropic GFP reporter virus for 2 h at room temperature; cells were cultured in R10 medium for 48 h and then analyzed by flow cytometry. LN sample numbers are shown at right. (B) Fresh disaggregated tonsil cells were stained, sorted, and then spinoculated with R5- or X4-tropic GFP reporter virus for 2 h at room temperature. Cell were cultured in R10 medium for 48 h and then analyzed by flow cytometry. The percentages of GFP⁺ cells are shown. Horizontal lines indicate medians.