FIG 3.

Viral phylogenetic relatedness between mother and child. Replication capacities were successfully determined for 41 MIPs, including 31 MIPs in the Durban laboratory and an additional 10 MIPs from an independent laboratory. Out of the 41 pairs, sequencing data were successful for 39 pairs. A maximum likelihood phylogenetic tree was constructed by using gag sequences, generated by Sanger and ultradeep sequencing, from 39 mother-infant pairs and the subtype C reference sequence. Sequences from the mothers are highlighted in red, and sequences from the infants are highlighted in blue. Viral sequences from the mothers and infants in each of the pairs clustered together, confirming the phylogenetic relatedness between viruses isolated from the mother and infant in each pair.