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Virulence of pH1N1 LAIV viruses. Six- to 8-week-old female C57BL/6 mice (n = 5) were infected i.n. with the indicated PFU of pH1N1 LAIV PAWT+/NS1WT− (A), PAMUT−/NS1WT− (B), PAWT+/NS1MUT+ (C), and PAMUT−/NS1MUT+ (D) viruses and then monitored daily for 2 weeks for body weight loss (left) and survival (right). Mice that lost 25% of their initial body weight were sacrificed. Data represent the means and SDs of the results determined for individual mice (n = 5).
