FIG 3.

Effects of Nlrc5 deletion in the lungs of IAV-infected mice. Mice were infected with 900 PFU of PR8 virus, and lungs were harvested on day 2 or 7 after infection. (A and B) Lung cytokine levels were determined on the indicated days after infection by ELISAs for IL-1β and IL-6. (C) Lung neutrophil numbers were determined by flow cytometry. (D to G) Flow cytometry examinations of MHC-I expression on total lung leukocytes (D and E), total spleen leukocytes (F), and total mediastinal lymph node (MdLN) leukocytes (G). The data are presented as geometric MFI. Data are representative of three or four independent experiments (n = 4 to 9 mice per genotype per experiment) and are means ± SEM. *, P < 0.05; ***, P < 0.001 (two-sided unpaired Student's t test).