Fig. 3.
Time-lapse MPM imaging of the dynamics of podocyte pseudocyst volume changes in vivo in PAN-treated Munich-Wistar-Fromter rat kidneys. Red: Plasma marker Alexa 594-albumin. Green/yellow: Lucifer yellow (LY) infused continuously into the carotid artery to label the Bowman’s space and to identify podocytes by negative labeling. Images taken 12 days after PAN treatment. Numerous albumin-containing (red) podocyte pseudocysts are visible in negatively labeled podocytes (asterisks). Compared to image taken at time zero (a), the volume of several pseudocysts increased or decreased within 30 s (b). c Time-dependent dynamic alterations in volume and albumin fluorescence intensity (Falb) of four different podocyte pseudocysts as indicated by color-matched arrows in b. Also see Supplement movie 1 showing the same preparation. Scale bar = 50 μm