(A) Shown are triple-color STORM images displaying AKAP150-mediated multi-channel complexes containing CaV1.2 and TRPV1 channels from NG sensory neurons isolated from WT mice. (B) Shown are dual-color STORM images showing discrete channel complexes containing CaV1.2, or TRPV1 channels from NG neurons isolated from AKAP150 KO mice. (C–D) Plotted are distributions of STORM nearest-neighbor distances for AKAP150/TRPV1, CaV1.2/TRPV1, and AKAP150/CaV1.2 from WT neurons (C) or CaV1.2 and TRPV1 from AKAP150 KO neurons (D), indicating intimate association of CaV1.2 and TRPV1 channels and AKAP150, in cells from WT, but not AKAP150, mice. n=4 and 5 cells.