FIGURE 3.

Both native and denatured rVRQ inhibit prion replication. PMCA amplification of a scrapie isolate (PG1361/05) was performed in triplicate in the presence of 1200 nM of rVRQ with or without denaturation or in the absence of any rPrP (as indicated). 6.7 µL of PK digested PMCA reaction was analyzed by western blotting using monoclonal antibody SHa31 (A). Following densitometry, signals were expressed as the percent inhibition, calculated using the no rPrP controls as 100% amplification (B). No significant difference in the inhibition levels between rVRQ and denatured rVRQ was observed (unpaired Student's t-test, p = 0.08). Molecular mass markers are indicated.