Table 6.
Mitochondrial % moving, distance moved, and speed in anterograde and retrograde direction for control, 3 day chronic, and Acute IOP elevation explants. Count = average number of mitochondria in imaging window (200μm × 100 μm). Mean ± standard deviation.
| Anterograde | Retrograde | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Experimental Groups | Samples (Movement Measured) |
Mitochondria Count |
Mitos Moving (%) |
Distance (μm) | Speed (μm/s) |
Samples (Movement Measured) |
Mitochondria Count |
Mitos Moving (%) |
Distance (μm) | Speed (μm/s) |
| Control-1 Minute | 4 (3) | 302 | 3.42 ± 1.69 | 11.30 ± 2.91 | 0.22 ± 0.10 | 4 (3) | 302 | 1.09 ± 0.70 | 10.63 ± 2.37 | 0.50 ± 0.12 |
| Control-10 Minutes | 4 (3) | 302 | 2.42 ± 1.82 | 12.92 ± 4.86 | 0.30 ± 0.11 | 4 (3) | 302 | 0.44 ± 0.10 | 10.31 ± 3.48 | 0.79 ± 0.33 |
| Glaucoma-1 Minute | 4 (1) | 214 | 5.14 | 13.64 ± 5.32 | 0.27 ± 0.11 | 4 (1) | 214 | 0.93 | 11.03 ± 0.49 | 0.46 ± 0.02 |
| Glaucoma-10 Minutes | 4 (1) | 214 | 0.93 | 12.10 ± 4.26 | 0.31 ± 0.04 | 4 (0) | 214 | |||
| Acute IOP: 10 mm Hg- 1 Minute | 21 (10) | 251 | 5.4 ± 4.48 | 13.18 ± 5.02 | 0.31 ± 0.13 | 21 (6) | 237 | 1.76 ± 2.00 | 11.81 ± 3.35 | 0.34 ± 0.16 |
| Acute IOP: 30 mmHg- 1 Minute | 21 (8) | 221 | 6.72 ± 4.00 | 13.21 ± 5.21 | 0.32 ± 0.13 | 21 (5) | 206 | 1.22 ± 0.97 | 11.08 ± 4.17 | 0.29 ± 0.11 |
| Acute IOP: 30 mmHg- 60 Minutes | 21 (4) | 222 | 5.35 ± 6.17 | 15.29 ± 5.95 | 0.30 ± 0.15 | 21 (4) | 202 | 1.62 ± 1.04 | 12.95 ± 4.49 | 0.31 ± 0.13 |