Table 1.

Minimal Inhibitory Concentrations and Potentiating Activities of Top Hits

	MIC WT, μM	MIC WT-Pore, μM	MIC ΔTolC, μM	MIC ΔTolC-Pore, μM	MPC4 NOV,a μM	MPC4 ERY, a μM	AcrAb binder	ΔTolC-Porec
NSC 60339	>200	200	12.5	12.5	25	12.5	yes	no
NSC 227186	>50	25	6.25	3.13	6.25	3.13	yes	no
NSC 33353	200	100	12.5	12.5	1.56	3.13	yes	no
NSC 305798	50	25–50	25	12.5	12.5	3.13	yes	no
NSC 50651	>50	3.13	6.25	0.8	1.56	6.25	yes	no
NSC 260594	>50	3.13	0.8	0.1	0.39	3.13	no	yes
NSC 354844	>100	6.25	50	6.25	1.56	NAd	yes	no
NSC 325014	25	3.13	12.5	3.13	3.1	0.39	no	no
NSC 207895	25	6.25	25	12.5	0.78	1.56	yes	no
NSC 56410	12.5	3.13	1.56	1.56	0.39	NA	no	yes
NSC 26980	3.13	3.13	0.19	0.19	0.39	0.39	no	yes

^aPotentiation in WT-Pore; NOV, novobiocin; ERY, erythromycin.

^bSee Figure S5 for experimental data and K_D values of selected hits.

^cPotentiation in efflux-deficient ΔTolC-Pore cells.

^dNA, no activity.