Skip to main content
. 2017 Jul 19;16(15):1440–1452. doi: 10.1080/15384101.2017.1338985

Figure 2.

Figure 2.

S109D mutation does not impair the ability of S67-phosphorylation of ARPP19 to activate Cdk1. A. WT-ARPP, S109A-ARPP and S109D-ARPP were incubated or not with recombinant Gwl in the presence of γS-ATP. The phosphorylation of WT-ARPP at S67 was visualized by western blot using an antibody directed against S67-phosphorylated ARPP (pS67-ARPP). Total ARPP19 was immunoblotted with an anti-GST antibody (GST-ARPP). B. Prophase-arrested oocytes were stimulated with progesterone (Pg) or injected with either unphosphorylated WT-ARPP (ARPP), S109A-ARPP and S109D-ARPP or S67-thiophosphorylated WT-ARPP, S109A-ARPP and S109D-ARPP (respectively pS67-ARPP, pS67-S109A-ARPP and pS67-S109D-ARPP). Meiosis resumption was followed by scoring the % of oocytes at GVBD as a function of time. C. Prophase-arrested oocytes were injected or not with p21Cip1 (Cip1) and then stimulated with progesterone (Pg) or by injecting S67-phosphorylated WT-ARPP, S109A-ARPP or S109D-ARPP (respectively pS67, pS67-S109A, pS67-S109D). Oocytes were collected at GVBD time. Cdk1 activation was monitored by western blotting phosphorylated MAPK (pMAPK) and Cdc27.