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. 2017 Jul 19;16(15):1440–1452. doi: 10.1080/15384101.2017.1338985

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© 2017 Taylor & Francis

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Figure 3. — S67-phosphorylated ARPP19 interacts with PP2A-B55δ independently of its S109 phosphorylation. A. Prophase-arrested oocytes were injected or not with p21^Cip1 (Cip1) and then injected with thiophosphorylated ARPP at either S109 (pS109), or S67 (pS67) or both residues (pS67-pS109). Oocytes were collected at the time of GVBD and ARPP19 proteins were GST-pulled down. GST-pulled down fractions were immunoblotted for PP2A-C ανδ B55δ subunits. Total ARPP19 was immunoblotted using a GST antibody (GST-ARPP). B. Prophase-arrested oocytes were induced to mature with progesterone (Pg) or by injecting S67-phosphorylated WT-, S109A- or S109D-ARPP (respectively pS67, pS67-S109A, pS67-S109D). Oocytes were collected at the time of GVBD and ARPP19 proteins were GST-pulled down. GST-pulled down fractions were immunoblotted for B55δ, PP2A-C subunits and total ARPP19 using a GST antibody (GST-ARPP).