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. Author manuscript; available in PMC: 2018 Aug 26.
Published in final edited form as: Biochem Biophys Res Commun. 2017 Jun 28;490(3):1074–1079. doi: 10.1016/j.bbrc.2017.06.168

Fig. 2. Autophosphorylation of Tyro3 occurs at tyrosine 756 and 723.

Fig. 2

(A) Schematic of truncated Tyro3 and immunoblotting of immunoprecipitated and total cellular GFP-tagged truncated Tyro3 mutants using the indicated antibodies. (B) Immunoblotting of immunoprecipitated GFP-tagged proteins and total cell lysate of NR6WT cells transiently cotransfected with the indicated plasmids. (C) Immunoblotting of endogenous Axl of fibroblasts NR6WT transfected by murine Axl siRNA for 48h. (D) Immunoblotting of immunoprecipitated exogenous WT Tyro3-eGFP from fibroblasts NR6WT transfected with Axl siRNA for 24h followed by further transfection of WT Tyro3 plasmid. (E) Immunoblotting of immunoprecipitated GFP-tagged ACTN4 and total cellular GFP-tagged Tyro3. Stained membrane stands for PVDF membrane that was stained for protein. Shown are representative images of three independent experiments.