TABLE 3.
qRT-PCR Evaluation of Gene Expression in the Male Rhesus Macaque Arcuate Nucleus
| AM
|
PM
|
||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Description | GenBank ID | Old (Control) | Young | Old HS | Old | P value |
| Steroid receptors | |||||||
| AR | Androgen receptor | AF092930 | 0.87 ± 0.10 | 1.26 ± 0.05* | 1.15 ± 0.04* | 1.24 ± 0.10* | 0.0052 |
| ESR1 | Estrogen receptor 1 | S71040 | 1.16 ± 0.25 | 0.98 ± 0.18 | 0.84 ± 0.08 | 0.84 ± 0.07 | 0.5705 |
| ESR2 | Estrogen receptor 2 | NM_001265821 | 0.86 ± 0.13 | 1.07 ± 0.09 | 0.94 ± 0.09 | 1.06 ± 0.06 | 0.4096 |
| PGR | Progesterone receptor | XM_001095317 | 0.89 ± 0.10 | 1.51 ± 0.08* | 1.09 ± 0.09 | 1.07 ± 0.25 | 0.0211 |
| Neuropeptides | |||||||
| KISS1 | Kisspeptin | AY823262 | 1.21 ± 0.18 | 1.94 ± 0.37 | 1.00 ± 0.20 | 1.83 ± 0.87 | 0.3570 |
| TAC3 | Tachykinin 3 | XM_001115535 | 0.44 ± 0.06 | 0.66 ± 0.11 | 0.36 ± 0.07 | 0.59 ± 0.25 | 0.3220 |
GeneChip array data were corroborated by qRT-PCR expression analysis. Values represent means ± SEMs. AM = morning necropsy; PM = evening necropsy; HS = 6 months of T and DHEA hormone supplementation.
*
P<0.05, ANOVA with post hoc Bonferroni adjustment, relative to the Old (Control) group.