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. 2017 Aug 10;17:186. doi: 10.1186/s12862-017-1011-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Estimate of the signatures of selection (d_N/d_S) in the PELPIII and TTS genes among selected Nicotiana spp. The evolutionary distances were computed using the Tamura-Nei method [79] and are in the units of the number of base substitutions per site. d_n/d_s ratios were calculated along each branch using the Nei-Gojobori method. The trees show negative selection (d_n/d_s < 1) in all branches. The color intensity indicates the d_n/d_s ratio. The evolutionary history was inferred using the Neighbor-Joining method [67] and the bootstrap test was performed for each tree (500 replicates; [21]). Bootstrap values are next to each node