Table 1.
Summary of the R. zambeziensis transcriptome assembly and annotation statistics
| Transcriptome statistics | Valuea |
|---|---|
| Transcriptome assembly statistics | |
| Total number of transcripts | 23,631 |
| Number of transcripts > 500 bp | 19,903 |
| Number of transcripts > 1 kb | 13,330 |
| Number of transcripts > 10 kb | 80 |
| Shortest transcript length (bp) | 201 |
| Longest transcript length (bp) | 17,108 |
| Mean length of transcripts (bp) | 1793.5 |
| Median length of transcripts (bp) | 1193 |
| Transcript N50 (bp) | 2807 |
| Total bases in assembly (Mb) | 42.4 |
| Ambiguous base calls (Ns) | 0 |
| GC content (%) | 49 |
| Number of non-redundant predicted proteins | 13,584 |
| Transcriptome annotation statisticsb | |
| BLASTx against NR | 12,756 (54.0%) |
| BLASTx against UniProtKB/TrEMBL | 16,451 (69.6%) |
| BLASTx against UniProtKB/Swiss-Prot | 10,572 (44.7%) |
| BLASTx against TSA-NR | 16,711 (70.7%) |
| BLASTx against Ixodes scapularis predicted peptides | 11,804 (50.0%) |
| BLASTx against EuKaryotic Orthologous Groups (KOG) | 9620 (40.7%) |
| BLASTx against AcariDB | 18,245 (77.2%) |
| Assigned with Gene Ontology (GO) termsc | 11,360 (48.1%) |
| Assigned with Enzyme Commission (EC) numbersc | 3493 (14.8%) |
| Assigned with KEGG orthology (KO) identifiersd | 4869 (20.6%) |
| Annotated in at least one database | 18,311 (77.5%) |
aValue indicating either the number of transcripts, proteins or bases, the transcript length or percentage, as indicated in the table
bNumber (and %) of transcripts annotated based on significant matches (E-value < E-05) against databases as detailed in the Methods section
cGO terms and EC numbers assigned with Blast2GO
dFrom the KEGG (Kyoto Encyclopedia of Genes and Genomes) Automatic Annotation Server (KAAS) using the I. scapularis genome