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. Author manuscript; available in PMC: 2017 Oct 10.
Published in final edited form as: Nat Genet. 2017 Apr 10;49(5):780–788. doi: 10.1038/ng.3838

Figure 1. Medulloblastomas, but not glioblastomas, show reliable transcriptome-based subgroup prediction.

Figure 1

(a) Unsupervised HCL using 1,000 high–SD transcripts of eight multi-region medulloblastoma (MB) samples combined with single biopsies (n=334) demonstrates tight clustering of matched multi-region MB samples across subgroups. (b) Box plot of the top 2000 SD-transcript values determined on an intra- and inter-tumor level in MB, HGG and RCC. (c) Principal component analysis (PCA) using 22 MB subgroup marker genes confirms a low degree of transcriptional intra-tumoral heterogeneity exemplified in MB3. Multi-region biopsy numbers of MB3 are indicated, and PCA was conducted with 103 single biopsy samples analyzed by NanoString. (d) Dot plot illustrating highly comparable marker gene expression in all multi-region biopsies for MB3. (e) Illustration showing GBM subtype and MB subgroup predictions based on Predictive Analysis of Microarrays (PAM) results. SHH subgroup affiliation of MB3 (marked with a *) was inferred based on NanoString results. Hashed circles are biopsies with <100% prediction confidence.

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