| Step | Temperature | Time | Number of cycles |
| Initial denaturation | 95 °C | 5 min | 1 |
| Denaturation | 98 °C | 20 sec |
34
|
| Primer annealing |
Primers melting temperature
60-75 °C
|
15 sec | |
| Extension | 72 °C | 30 sec/kb | |
| Final extension | 72 °C | 5 min | 1 |
| cooling | 12 °C | hold | 1 |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.