| Step | Temperature | Time | Number of cycles |
| Initial denaturation | 94 °C | 30 sec | 1 |
| Denaturation | 94 °C | 30 sec |
34
|
| Primer annealing |
Primers melting temperature
55-65 °C
|
30 sec | |
| Extension | 72 °C | 60 sec/kb | |
| Final extension | 72 °C | 5 min | 1 |
| cooling | 12 °C | hold | 1 |
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