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. Author manuscript; available in PMC: 2017 Aug 11.
Published in final edited form as: Methods Mol Biol. 2017;1619:81–101. doi: 10.1007/978-1-4939-7057-5_7

Fig. 2.

Fig. 2

(a) Approximate depletion efficiencies of medium-abundance proteins captured by the SuperMix column (see Ref. [10]). The IgY 14/SuperMix flow-through (FT) and SuperMix Bound (B) fractions from triplicate depletions of three plasma pools (n=9) were quantitated using MaxQuant. % Depletion Efficiencies (%DE) were calculated for each protein based on normalized MS intensities of the bound fraction and flow-through fractions i.e. %DE = [B/(B + FT)] × 100. (b) Depletion efficiencies of the residual high abundance proteins targeted by the IgY 14 column, remaining after IgY 14 /SuperMix depletion. Averages and standard error bars are shown in both panels. l.c.= light chain.