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. Author manuscript; available in PMC: 2017 Aug 11.
Published in final edited form as: Methods Mol Biol. 2017;1619:81–101. doi: 10.1007/978-1-4939-7057-5_7

Table 2. FPLC Timetable for IGY 14/SuperMix Tandem Column Depletion (Single Elution).

Cycle Time (min) 1× Dilution Buffer 1× Stripping Buffer 1× Neutralization Buffer Flow Rate (ml/min) Max Pressure (MPa)
Sample Loading 0 100 0 0 0.5 2.5
Wash 6 100 0 0 0.5 2.5
Wash 24 100 0 0 1.5 2.5
Elution 34 0 100 0 1.5 2.5
Neutralization 54 0 0 100 1.5 2.5
Re-equilibration 64 100 0 0 1.5 2.5
End 84 0 0 0 0 2.5