Figure 4.

(a–h) Calcein AM (green, live cells) and ethidium homodimer (red, dead cells) staining of C2C12 premyoblasts 24 h after treated with media containing ^CLK₃ ([^CLK₃] = 27.5 μM) at different irradiation times: (a) 0 h, (b) 8 h, (c) 24 h, (d) 72 h, (e) 144 h. (f) Nonirradiated infinitely long fibers (long, 0 h irradiation), (g) irradiated infinitely long fibers (long, 144 h irradiation) and (h) tissue culture plate as control experiments. (i) Cell viability was quantified as percentage of live cells (green) in total number of cells (green + red). ****p < 0.0001, *p < 0.05 (0 h irradiation: nonirradiated ^CLK₃. 8 h irradiation: ^CLK₃ with 8 h of irradiation. 24 h irradiation: ^CLK₃ with 24 h of irradiation. 72 h irradiation: ^CLK₃ with 72 h of irradiation. 144 h irradiation: ^CLK₃ with 144 h of irradiation. Infinitely long, 0 h irradiation: nonirradiated infinitely long fibers of ^CLK₃. Infinitely long, 144 h irradiation: infinitely long fibers of ^CLK₃ with 144 h of irradiation. Control: cells in a tissue culture plate. (j, k) Phase micrographs of C2C12 premyoblasts 24 h after treated with media containing ^CLK₃ ([^CLK₃] = 27.5 μM), irradiated for (j) 0 h or (k) 144 h. Arrows indicate dead cells.