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. 2017 Aug 11;12(8):e0181784. doi: 10.1371/journal.pone.0181784

Table 1. List of identified genes involved in the saponin biosynthesis pathway with their unigene.

Kyoto Encyclopedia of Genes and Genomes (KEGG) enzyme commission (EC) number and read length (base pair, bp).

Gene name Unique sequence KEGG enzyme commission (EC) Read length (bp)
acetyl-CoA acetyltransferase CL6820.Contig3 [EC:2.3.1.9] 685
hydroxymethylglutaryl-CoA-synthase CL2608.Contig3 [EC:2.3.3.10] 1827
hydroxy methylglutaryl-CoA-reductase Unigene29227 [EC:1.1.1.34] 1926
mevalonate kinase CL3170.Contig2 [EC:2.7.1.36] 1256
phosphomevalonate kinase Unigene11622 [EC:2.7.4.2] 2283
diphosphomevalonate decarboxylase Unigene27463 [EC:4.1.1.33] 1584
isopentenyl diphosphate isomerase 1 Unigene3676 [EC:5.3.3.2] 871
geranyl diphosphate synthase 1 Unigene37026 [EC:2.5.1.1 2.5.1.10 2.5.1.29] 206
farnesyl diphosphate synthase Unigene27333 [EC:2.5.1.1 2.5.1.10] 1394
squalene synthase 1 Unigene26049 [EC:2.5.1.21] 524
squalene epoxidase monooxygenase 1 CL5543.Contig2 [EC:1.14.14.17] 1922
squalene epoxidase 3 CL5543.Contig3 [EC:1.14.14.17] 302
cycloartenol synthase 1 CL2408.Contig1 [EC:5.4.99.8] 2718
cycloartenol-C-24-methyltransferase Unigene11966 [EC:2.1.1.41] 1406
methylsterol monooxygenase Unigene21326 [EC:1.14.13.72] 1259
cycloeucalenol cycloisomerase CL6464.Contig1 [EC:5.5.1.9] 1252
obtusifoliol (Sterol) 14-alpha demethylase Unigene27267 [EC:1.14.13.70] 1826
delta(14)-sterol reductase Unigene28058 [EC:1.3.1.70] 1358
7-dehydrocholesterol reductase Unigene27272 [EC:1.3.1.21] 1669
delta-7-sterol 4-alpha-methyl-oxidase Unigene39702 [EC:1.14.13.72] 152
24-methylenesterol C-methyltransferase 2 CL4881.Contig1 [EC:2.1.1.143] 168
delta-7-sterol-C5(6)-desaturase CL1225.Contig2 [EC:1.14.19.20] 639
UDP-sterol-glucosyltransferase Unigene10659 [EC:2.4.1.173] 224
UDP-sterol-glucosyltransferase CL141.Contig10 [EC:2.4.1.173] 1000
UDP-sterol-glucosyltransferase Unigene26213 [EC:2.4.1.173] 736
glycosyltransferase, family 43 CL1481.Contig2 [EC:2.4.1.17] 1568
UDP-sterol-glucosyltransferase CL141.Contig4 [EC:2.4.1.173] 1657
UDP-glycosyltransferase Unigene24555 [EC:2.4.1.17] 441
UDP-glycosyltransferase Unigene15513 [EC:2.4.1.17] 294
UDP-glucosyltransferase73B5 Unigene27343 [EC:2.4.1.17] 1509
UDP-glucosyltransferase71B1 CL2556.Contig3 [EC:2.4.1.17] 227
UDP-glucosyltransferase 73C6 Unigene18419 [EC:2.4.1.-] 535
CYP72A5 CL2624.Contig2 [EC:1.14.-.-] 1430
CYP709B2 Unigene21995 [EC:1.14.-.-] 1794
CYP734A1 CL2624.Contig3 [EC:1.14.-.-] 1392
CYP72A9 CL2624.Contig4 [EC:1.14.-.-] 1177
CYP94D2 CL3985.Contig1 [EC:1.14.-.-] 1364
CYP71B31 Unigene25910 [EC:1.14.-.-] 430
CYP81D5 CL5820.Contig2 [EC:1.14.-.-] 736
CYP94C1 Unigene12533 [EC:1.14.-.-] 1867
CYP706A6 Unigene416 [EC:1.14.-.-] 1024
CYP710A1 Unigene30597 [EC:1.14.19.41] 467
beta-glucosidase 7 Unigene27678 [EC:3.2.1.58] 1402
beta glucosidase 13 CL5385.Contig1 [EC:3.2.1.21] 193
beta-glucosidase 17 Unigene27758 [EC:3.2.1.58] 1674
beta glucosidase 42 CL534.Contig1 [EC:3.2.1.21] 841
glycosyl hydrolase 9A1 CL28.Contig2 [EC:3.2.1.-] 2243
glycosyl hydrolase 9B5 CL1599.Contig3 [EC:3.2.1.-] 282
glycosyl hydrolase 9B7 CL1599.Contig2 [EC:3.2.1.-] 744
glycosyl hydrolase family 47 Unigene34243 [EC:3.2.1.113] 432