Fig. 6.

cAMP/PKA signaling mutations in P. pastoris clones adapted to minimal growth medium (BMD). a Occurrence of mutations grouped by strain background. The occurrence of a particular mutation is indicated by color (yellow to red for the presence of a mutation in each individual clone). A blank square indicates the absence of a mutation. b Enzymatic steps catalyzed by the mutated genes IRA1 and PDE2. c Fitness effect of a single IRA1 mutation in an evolved OCH1 clone (clone ∆OCH1 BMD G2b Tables S13-S15). Values represent averages +/− standard deviation; n = 4; paired Student’s T-test values * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001