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Effect of PAS-chr3_0669 overexpression in the ancestral strains. The effect of overexpression of the GAL4-like gene in comparison to an empty vector strain is shown for all ancestral strains in (a) YPD and YPDN rich media (b) BMD and BMDN minimal media (c) rich and minimal methanol growth media, YPM and BMM and (d) rich glycerol growth media, YPG and YPGN. Values represent averages +/− standard deviation; n = 4; paired Student’s T-test values * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001
