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. 2017 Aug 10;48:41. doi: 10.1186/s13567-017-0445-2

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of the HP-PRRSV TRSs on EGFP expression in Marc-145 cells infected with rHP-PRRSVs. Marc-145 cells were infected at an MOI of 0.01 and cultured until 60% of cells showed the cytopathic effect (CPE). For western blot analysis of EGFP production, total proteins were collected from virus-infected cells, electrophoresed, transferred to a polyvinylidene difluoride (PVDF) membrane, and immunostained using a mouse anti-GFP monoclonal antibody, anti-α-Tubulin antibody and anti-PRRSV N protein as a loading control.