Fig. 3.

Therapeutic effects of Bz and Cyc on serum and renal histopathology of 14-week-old and 22-week-old MRL/lpr mice. a Change of serum immunoglobulin subclasses and titer of anti-dsDNA antibody after Bz or Cyc treatment. b Representative histopathology of glomerulonephritis: (upper panel) hematoxylin–eosin staining (×100) and (lower panel) immunofluorescent analysis of glomerular lesions stained with FITC-anti IgG antibody. Cellular infiltrates and glomerular deposition of IgG were reduced in Bz-treated and Cyc-treated mice compared with controls. c Histopathological analysis of glomerulonephritis after Bz or Cyc treatment. Values expressed as median with quartile range. Statistical analysis performed using paired t test (a) and one-way ANOVA analyses with Turkey post-hoc test (c). In the control group, 19 mice aged 14 weeks were treated and 17 of them survived at 22 weeks of age (two mice died). In the Bz group, 27 mice aged 14 weeks were treated but only 16 mice survived at 22 weeks of age (11 mice died). However, in the Cyc group, 20 mice aged 14 weeks were treated and all survived at 22 weeks of age (no mice died). anti-dsDNA anti-double-stranded DNA, A.U. arbitrary units, Bz bortezomib, Con control, Cyc cyclophosphamide