Fig 3. Comparison of male and female C57BL/6 bone marrow immune cell Wnt10b expression.

Bone marrow (BM) was isolated from the femora of male (n = 22) and female (n = 5) C57BL/6 adult mice and analyzed by flow cytometry. Wnt10b MFI, % of cell type and % of total bone marrow was calculated. a) Female total BM expressed significantly higher levels of Wnt10b compared to males with significant cell-type specific sex-differences identified. b) Relative contribution of cell-types investigated to the Wnt10b⁺ population in males and females. c) Female total BM had a higher % of Wnt10b⁺ cells compared to males; cell-type specific differences in Wnt10b expression profiles were observed between the sexes. Statistical analysis performed by student’s t-test; *p<0.05 and **p<0.01.