Fig 4. Regulation of male C57BL/6 bone marrow Wnt10b expression by TNFα.

Bone marrow (BM) was isolated from the femora of male WT (n = 23) and TNF^ko (n = 9) C57BL/6 mice and analyzed by flow cytometry. Wnt10b MFI, % of cell type and % of total bone marrow was calculated. a) Ablation of TNFα significantly increased male BM and BM immune cell Wnt10b cellular expression. b) Relative contribution of cell-types investigated to the Wnt10b⁺ population in male WT and TNF^ko mice. c) Numbers of Wnt10b⁺ cells as a % of cell type and total BM were significantly reduced in the TNF^ko mice compared to WT. Statistical analysis performed by student’s t-test; *p<0.05 and **p<0.01.