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. 2017 Aug 11;12(8):e0181979. doi: 10.1371/journal.pone.0181979

Fig 7. Effect of estrogen-deficiency on Balb/c bone marrow Wnt10b expression.

Fig 7

Female Balb/c mice (12 weeks) underwent either sham or ovariectomy (OVX) surgery. Mice were harvested at 4 (n = 13), 6 (n = 4) or 8 (n = 10) weeks and bone marrow (BM) isolated from a femur and analyzed by flow cytometry. Wnt10b MFI, % of cell type and % of total bone marrow was calculated. OVX lowered total BM Wnt10b cellular expression but had no effect on any of the cell types investigated. % of Wnt10+ cells were significantly reduced in the OVX cohort across the time course compared to the sham control. Statistical analysis performed by 2-way ANOVA with Sidak post- test; *p<0.05.