Fig 4. Paired co-injection experiments indicated chromatin accessibility information could be applied to improve gRNA designs.

(A) Dot plot of mutation rates for gRNAs targeting on OC regions (blue), and gRNAs targeting on CC regions (red). (B) Barplot of the mutation rate differences between paired co-injected gRNAs. (C) Boxplot of mutation rates of gRNA targeting on OC region (blue) and CC region (red), paired t-test p-value: 0.0199. (D) Summary of discretized mutation rates for gRNAs targeting on OC regions and CC regions separately.