Figure 2.

CCR7 enhances the angiogenic capacity of esophageal squamous carcinoma cells in vitro. A. Western blotting analysis of CCR7 protein expression in ECA109-vector, and ECA109-CCR7 cells; α-Tubulin was used as a loading control. B. Cell viability was determined by MTT proliferation assay after ECA109-vector and ECA109-CCR7 cells cultured for the indicated days. C. Representative images of cell morphology in ECA109 cells were transfected with vector or CCR7. D. HUVEC viability was determined by MTT proliferation assay after cells after incubation in culture supernatants derived from the indicated cells. E. Representative images (left) and quantification (right) of the number of migrated HUVEC cells after incubation in culture supernatants derived from the indicated cells in the migration assay. F. Representative images (left) and quantification (right) of capillaries formed in the CAM assay when stimulated by culture supernatants derived from the indicated cells. G. Western blotting analysis of VEGF-A and VEGF-C expression in the indicated cells. Data is mean ± SD of three independent experiments; for indicated comparisons, *P < 0.05, **P < 0.01.