Figure 3.

Knockdown of CCR7 reduces the angiogenic capacity of esophageal squamous carcinoma cells in vitro. A. Western blotting analysis of CCR7 protein expression in CCR7-siRNA-transduced Eca109 cell line (shown as CCR7-RNAi) and the corresponding control cells (shown as RNAi-Vector); α-Tubulin was used as a loading control. B. Cell viability was determined by MTT proliferation assay after CCR7-siRNA-transduced Eca109 and Vector-siRNA-transduced Eca109 cells cultured for the indicated days. C. Representative images of cell morphology in Eca109 cells were transfected with Vector-siRNA or siRNA-CCR7. D. HUVEC viability was determined by MTT proliferation assay after cells after incubation in culture supernatants derived from the indicated cells. E. Representative images (left) and quantification (right) of the number of migrated HUVEC cells when incubated in culture supernatants derived from the indicated cells in the Transwell migration assay. F. Representative images (left) and quantification (right) of neovessels formed in the CAM assay when stimulated by culture supernatants derived from the indicated cells. G. Western blotting analysis of VEGF-A and VEGF-C expression in the indicated cells. Data is mean ± SD of three independent experiments; for indicated comparisons, *P < 0.05, **P < 0.01.