Figure 4.

CCR7 promotes NF-κB transcriptional activity. A. Luciferase reporter assay of NF-κB transcriptional activity in CCR7-overexpressing or silenced cells expressed relative to the respective control cells. B. Western blotting analysis of the expression of phosphorylated IKK (p-IKK), total IKK, phosphorylated IκBα (p-IκBα) and total IκBα; α-Tubulin was used as a loading control. C. Western blotting analysis of the expression of genes downstream of NF-κB in the indicated cells; transcript levels were normalized to α-Tubulin. D. ELISA assay was conducted to evaluate the TNF-α, IL-6, IL-8, and TGF-β secretion in the indicated cells. Data is mean ± SD of three independent experiments; for indicated comparisons, *P < 0.05, **P < 0.01.