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. 2017 Jul 15;9(7):3360–3373.

Figure 3.

Figure 3

Sirt3 overexpression suppressed Dox-elevated Bnip3 expression and disrupted mitochondrial Cox1-Ucp3 complexes in vivo and in vitro. (A)Representative immunoblots and averaged data of Bnip3 protein expression in hearts from AdNull- or AdSirt3-infected rats with or without Dox treatment. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal control. (B) Representative immunoblots and averaged data of Bnip3 protein expression in AdNull- or AdSirt3-infected cardiomyocytes with or without Dox treatment. GAPDH was used as an internal control. (C) Immunoprecipitation analysis of the Cox1-Ucp3 complex. Protein lysate derived from hearts from AdNull- or AdSirt3-infected rats with or without Dox treatment was probed with an antibody directed against Cox1 (2 μg/mL) and blotted with an antibody directed against Ucp3. (D) Immunoprecipitation analysis of the Cox1-Ucp3 complex in AdNull- or AdSirt3-infected cardiomyocytes with or without Dox treatment analyzed as in (C). Data are presented as mean ± SEM. *P<0.05, **P<0.01 versus the corresponding vehicle group; #P<0.05, ##P<0.01 versus the corresponding AdNull group.