Figure 3.

Quinacrine treatment permanently cures ScN2a cells. Anti-PrP immunoblots of protease-digested ScN2a cell lysates treated with 0.2–0.8 μM quinacrine for 6 days (left) and then left without treatment and weekly splitting for 3 weeks. The lack of reappearance of protease-resistant PrP^Sc after discontinuation of treatment demonstrates that quinacrine permanently cures ScN2a cells. Molecular mass markers at the right of the immunoblot correspond to 37 kDa (upper band) and 20 kDa (lower band).