Figure 2. Single fly αβ-KC sequencing suggests transposon hovering.
(a) Schematic of the experimental approach. Six individual flies were processed independently. The circular plot shows the gDNA sequencing coverage of mCherry positive αβ-KCs (red trace) and mCherry negative cells from the rest of the brain (blue trace), on chromosome 2R from one representative individual fly. The schematic (top right) depicts the 4 fruit fly chromosome pairs. Chromosome 2R, which is the source data in the circular plot, is highlighted in black. Schematic fly brain (bottom right) indicates the color scheme; αβ-KCs (red), the rest of the brain (blue). Sequencing read alignments on other regions of the gDNA exhibited a similar coverage (data not shown). (b) Plot of a representative example of a germline transposon insertion that was found on chromosome 2L in each of the 6 individual flies and that is absent in the Drosophila melanogaster reference genome (Release 5.57). Putative new insertions were found at loci, which were approximately 10 kb up- and downstream of the germline insertion site of the same transposon type. Dark red diamonds represent the germline insertion of the transposon Doc, which was found in each of the 12 samples (αβ-KCs and the rest of the brain), and light red diamonds represent putative somatic Doc insertions. The genomic location of the turtle gene is shown below in blue. Boxes indicate exons and lines intronic regions of the gene. Schematic fly brain represents the color code used for this panel.
Figure 2—figure supplement 1. Total number of putative non-reference somatic TE insertions from 12 samples with 1, 2, 3–9 and more than 10 diagnostic reads.
