Table 3.

Sequences of oligonucleotide primers for real-time RT-PCR.

DOI: http://dx.doi.org/10.7554/eLife.28527.020

A. Sequences of oligonucleotide primers for real-time RT-PCR in human tissues.
Gene	Forward primer	Reverse primer	Accession no.
NPGL	GGAACCATGGCTTAGGAAGG	CCTTAGGAGCTGAGAATATGTA	NM_001102659.1
ACTB	GGCACCACACCTTCTACAAT	AGGTCTCAAACATGATCTGG	NM_001101.3

B. Sequences of oligonucleotide primers for real-time RT-PCR in rat tissues.
Gene	Forward primer	Reverse primer	Accession no.
Npgl	GGAACCATGGCTTAGGAAGG	TCTAAGGAGCTGAGAATATGCA	LC003309
Acc	GAGGTGGATCAGAGATTTCA	TTCAGCTCTAACTGGAAAGC	NM_022193.1
Fas	AGGATGTCAACAAGCCCAAG	ACAGAGGAGAAGGCCACAAA	NM_017332.1
Scd1	TGAAAGCTGAGAAGCTGGTG	CAGTGTGGGCAGGATGAAG	NM_139192.2
Gpat1	CAGCGTGATTGCTACCTGAA	CGGAAGGTGTGGACAAAGAT	NM_017274.1
Adpn	GGGCTACGCTATGTCTGAGC	GAGACTGCACACGAAGGTGA	NM_001282324.1
Cpt1a	GGATGGCATGTGGGTAAAAG	TACTGACACAGGCAGCCAAA	NM_031559.2
Atgl	GCTGCAAGTGGGTTTTTGAT	GTGAACGGTAAGGCACAGGT	NM_001108509.2
Hsl	GAGACGGAGGACCATTTTGA	CGGAGGTCTCTGAGGAACAG	NM_012859.1
Srebp1	TCACAGATCCAGCAGGTCCCC	GGTCCCTCCACTCACCAGGGT	NM_001276707.1
Lpl	TCTCCTGATGATGCGGATTT	CAACATGCCCTACTGGTTTC	NM_012598.2
Cd36	GAGGTCCTTACACATACAGAGTTCGTT	ACAGACAGTGAAGGCTCAAAGATG	NM_031561.2
Fatp1	GCGGCGTTCGGTGTGTAC	GCACGCGGATCAGAACAGA	NM_053580.2
Slc2a2	GACATCGGTGTGATCAATGC	TGTCGTATGTGCTGGTGTGA	NM_012879
Slc2a4	CCTCCAGGATGAAGGAAACA	GGGAGAAAAGCCCATCTAGG	NM_012751
Gapdh	CGGCAAGTTCAACGGCACAG	ACTCCACGACATACTCAGCAC	NM_017008.4
Gk	TTGAGACCCGTTTCGTGTCA	AGGGTCGAAGCCCCAGAGT	NM_001270850.1
L-Pk	TGATGATTGGACGCTGCAA	GAGTTGGTCGAGCCTTAGTGATC	NM_012624.3
Npy	TATCCCTGCTCGTGTT	GATTGATGTAGTGTCGCAGA	NM_012614.2
Agrp	GCAGACCGAGCAGAAGATGT	GACTCGTGCAGCCTTACACA	NM_033650.1
Pomc	TAAGAGAGGCCACTGAACAT	GTCTATGGAGGTCTGAAGCA	NM_139326.2
Actb	GGCACCACACTTTCTACAAT	AGGTCTCAAACATGATCTGG	NM_031144.3
Rps18	AAGTTTCAGCACATCCTGCGAGTA	TTGGTGAGGTCAATGTCTGCTTTC	NM_213557.1

C. Sequences of oligonucleotide primers for real-time RT-PCR in mouse tissues.
Gene	Forward primer	Reverse primer	Accession no.
Npgl	GGAACCATGGCTTAGGAAGG	TCTAAGGAGCTGAGAATATGCA	LC088498
Actb	GGCACCACACCTTCTACAAT	AGGTCTCAAACATGATCTGG	NM_007393.4