FIGURE 2. Role of AMPAR activity in homocysteine-induced neuronal cell death.
Neuronal cultures were treated with L-homocysteine (50 μM, 18 h) in the absence or presence of NBQX (10 μM) or CNQX (50 μM) that were added 30 min after the onset of L-homocysteine treatment and then stained with Hoechst 33342, 18 h later. Representative photomicrographs show pyknotic DNA stained with Hoechst 33342 (indicated with arrows). Percentage of neurons with pyknotic nuclei is represented as mean ± SEM (n = 1500 cells/condition from 4 experiments). *p < 0.001 from control; #p < 0.05 from 18 h homocysteine treatment.