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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: J Neurochem. 2017 Jun 29;142(4):560–573. doi: 10.1111/jnc.14078

FIGURE 3. Homocysteine regulates surface expression of GluA2-AMPAR subunit.

FIGURE 3

(A–C) Neuron cultures were treated with 50 μM of L-homocysteine (L-Hcy) for 4 h. (A, B) L-homocysteine treated cells were subjected to biotinylation of surface proteins followed by immunoblot analysis of the purified biotinylated proteins using (A) anti-GluA1 (upper panel) or (B) anti-GluA2 (upper panel) antibody. Equal amounts of protein from total cell extracts (input lysate) were also analyzed by immunoblotting using (A) anti-GluA1 (lower panel) or (B) anti-GluA2 (lower panel) antibody. (C) An equal amount of protein from total cell lysates was assessed for GluA2 phosphorylation at Ser880 by immunoblot analysis using anti-phospho-GluA2-S880 antibody (p-GluA2; upper panel). Total GluA2 was also analyzed to indicate equal protein loading (lower panel). (A–C) Quantitative analysis of GluA1 and GluA2 surface expression as well as GluA2 phosphorylation at Ser880 is represented as mean ± SEM (n = 4). *p < 0.001 from untreated control.